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    產(chǎn)品展示 / products 您的位置:網(wǎng)站首頁 > 產(chǎn)品展示 > 細胞庫 > 細胞系 > 人肝癌細胞HepG2
    人肝癌細胞HepG2

    人肝癌細胞HepG2

    簡要描述:青旗(上海)生物技術(shù)發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術(shù)為主的研發(fā)、生產(chǎn)、培訓(xùn)為一體的綜合化產(chǎn)業(yè)平臺,在標準化細胞庫建立及細胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營原代細胞、細胞系、ELISA試劑盒、感受態(tài)細胞和HPLC檢測等科研產(chǎn)品與服務(wù)。我們秉承對用戶負責的態(tài)度,以對科研的高度嚴謹,以嚴格的質(zhì)量控制,為廣大生物醫(yī)學(xué)科研用戶提供更優(yōu)質(zhì)的服務(wù)!

    更新時間:2021-05-24

    廠商性質(zhì):生產(chǎn)廠家

    瀏覽次數(shù):709

    詳情介紹
    品牌其他品牌貨號BFN60800692
    規(guī)格T25培養(yǎng)瓶x1 1.5ml凍存管x2供貨周期現(xiàn)貨
    主要用途僅供科研應(yīng)用領(lǐng)域醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)

    細胞名稱

    人肝癌細HepG2                  

    img1

    貨物編碼

    BFN60800692

    產(chǎn)品規(guī)格

    T25培養(yǎng)x1

    1.5ml凍存x2

    細胞數(shù)量

    1x10^6

    1x10^6

    保存溫度

    37

    -198

    運輸方式

    常溫保溫運輸

    干冰運輸

    安全等級

    1

    用途限制

    僅供科研用途               1類

     

    培養(yǎng)體系

    DMEM高糖培養(yǎng)基Hyclone+10%胎牛血清Gibco+1%雙抗Hyclone

    培養(yǎng)溫度

    37

    二氧化碳濃度

    5%

    簡介

    人肝癌細HepG2細胞來源于一15歲的白人少年的肝癌組織。該細胞表達甲胎蛋白、白蛋白α-2-巨球蛋白α-1-抗胰蛋白酶、轉(zhuǎn)鐵蛋白α-1-抗凝乳蛋白酶、結(jié)合珠蛋白、銅藍蛋白、纖溶酶原、補C4C3激活物、纖維蛋白原、α-1酸性糖蛋白α-2-HS-糖蛋白、β-脂蛋白、視黃醇結(jié)合蛋白;表達胰島素受體和胰島素樣生長因IGF的受體;該細胞具3--3-甲酰輔A還原酶和肝甘油三酯脂肪酶的活性。目前尚未證明該細胞中HBV基因組。 人肝癌細HepG2細胞由青旗(上海)生物技術(shù)發(fā)展有限公司2019年引種ATCCHB-8065)

    注釋

    Problematic cell line: Misidentified. Originally thought to be a hepatocellular carcinoma cell line but shown to be from an hepatoblastoma (PubMed=19751877).

    Part of: Cancer Cell Line Encyclopedia (CCLE) project.

    Part of: ENCODE project common cell types; tier 1.

    Part of: JFCR45 cancer cell line panel.

    Part of: MD Anderson Cell Lines Project.

    Part of: TCGA-110-CL cell line panel.

    Doubling time: ~50-60 hours (DSMZ).

    Omics: Deep antibody staining analysis.

    Omics: Deep exome analysis.

    Omics: Deep phosphoproteome analysis.

    Omics: Deep proteome analysis.

    Omics: Deep RNAseq analysis.

    Omics: DNA methylation analysis.

    Omics: Genome sequenced.

    Omics: H3K27ac ChIP-seq epigenome analysis.

    Omics: H3K27me3 ChIP-seq epigenome analysis.

    Omics: H3K36me3 ChIP-seq epigenome analysis.

    Omics: H3K4me1 ChIP-seq epigenome analysis.

    Omics: H3K4me2 ChIP-seq epigenome analysis.

    Omics: H3K4me3 ChIP-seq epigenome analysis.

    Omics: H3K79me2 ChIP-seq epigenome analysis.

    Omics: H3K9ac ChIP-seq epigenome analysis.

    Omics: H3K9me3 ChIP-seq epigenome analysis.

    Omics: H4K20me1 ChIP-seq epigenome analysis.

    Omics: Metabolome analysis.

    Omics: Protein expression by reverse-phase protein arrays.

    Omics: Secretome proteome analysis.

    Omics: SNP array analysis.

    Omics: Transcriptome analysis.

    Omics: Virome analysis using proteomics.

    STR信息

    AmelogeninXYCSF1PO1011D13S3179,13;D16S5391213;D18S5113,14;D19S43315.2;D21S112931;D2S133819,20D3S135815,16;D5S8181112;D7S82010D8S117915,16FGA22,25;TH019;TPOX8,9;vWA17;

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    驗收細胞注意事 

    1、收到人肝癌細HepG2細胞,請查看瓶子是否有破裂,培養(yǎng)基是否漏出,是否渾濁,如有請盡快聯(lián)系 

    2、收到人肝癌細HepG2細胞,如包裝完好,請在顯微鏡下觀察細胞,由于運輸過程中的問題,細胞培養(yǎng)瓶中的貼壁細胞有可能從瓶壁中脫落下來,顯微鏡下觀察會出現(xiàn)細胞懸浮的情況,出現(xiàn)此狀態(tài)時,請不要打開細胞培養(yǎng)瓶,應(yīng)立即將培養(yǎng)瓶置于細胞培養(yǎng)箱里靜 3-5 小時左右,讓細胞先穩(wěn)定下,再于顯微鏡下觀察,此時多數(shù)細胞會重新貼附于瓶壁。如細胞仍不能貼壁,請用臺盼藍染色法鑒定細胞活力,如臺盼藍染色證實細胞活力正常請按懸浮細胞的方法處理。 

    3、收到人肝癌細HepG2細胞后,請鏡下觀察細胞,用恰當方式處理細胞。若懸浮的細胞較多,請離心收集細胞,接種到一個新的培養(yǎng)瓶中。棄掉原液,使用新鮮配制的培養(yǎng)基,使用進口胎牛血清。剛接到細胞,若細胞不多 血清濃度可以加 15%去培養(yǎng)。若細胞迏 80% ,血清濃度還是 10。 

    4、收到人肝癌細HepG2細胞時如無異常情 ,請在顯微鏡下觀察細胞密度,如為貼壁細胞,未超80%匯合度時,將培養(yǎng)瓶中培養(yǎng)基吸出,留 5-10ML 培養(yǎng)基繼續(xù)培養(yǎng):超 80%匯合度時,請按細胞培養(yǎng)條件傳代培養(yǎng)。如為懸浮細胞,吸出培養(yǎng)液,1000 轉(zhuǎn)/分鐘離 3 分鐘,吸出上清,管底細胞用新鮮培養(yǎng)基懸浮細胞后移回培養(yǎng)瓶。 

    5、將培養(yǎng)瓶置 37培養(yǎng)箱中培養(yǎng),蓋子微微擰松。吸出的培養(yǎng)基可以保存在滅菌過的瓶子里,存放 4冰箱,以備不時之需。 

    624 小時后,人肝癌細HepG2細胞形態(tài)已恢復(fù)并貼滿瓶壁,即可傳代。(貼壁細胞)將培養(yǎng)瓶里的培養(yǎng)基倒去, 3-5ml(以能覆蓋細胞生長面為準PBS  Hanks液洗滌后棄去。 0.5-1ml 0.25% EDTA 的胰酶消化,消化時間以具體細胞為準,一 1-3 分鐘,不超 5 分鐘??梢苑?/span>37培養(yǎng)箱消化。輕輕晃動瓶壁,見細胞脫落下來,加 3-5ml 培養(yǎng)基終止消化。用移液管輕輕吹打瓶壁上的細胞,使之*脫落,然后將溶液吸入離心管內(nèi)離心,1000rpm/5min。棄上清,視細胞數(shù)量決定分瓶數(shù),一般一傳二,如細胞量多可一傳三,有些細胞不易傳得過稀,有些生長較快的細胞則可以多傳幾瓶,以具體細胞和經(jīng)驗為準。(懸浮細胞)用移液管輕輕吹打瓶壁,直接將溶液吸入離心管離心即可。 

    7、貼壁細 ,懸浮細胞。嚴格無菌操作。換液時,換新的細胞培養(yǎng)瓶和換新鮮的培養(yǎng)液,37,5%CO2 培養(yǎng)。

     

    特別提醒 原瓶中培養(yǎng)基不宜繼續(xù)使用,請更換新鮮培養(yǎng)基培養(yǎng)。



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